배양된 사람 조골세포 분화에 사이클로스포린 A가 미치는 영향
The Effect of Cyclosporin A on Differentiation of Cultured Normal Human Osteoblast
이종헌,
소속 상세정보
이종헌 ( Lee Chong-Heon ) - 단국대학교 치과대학 구강병리학교실
KMID : 0829220110350010013
Abstract
Cyclosporin A(CsA) as immunosuppressive drug is used to prevent immune reactions after organ transplant. And also It is reported that the effect of CsA on osteoblast differentiation has been controversial according to dosage. The purpose of this study was to examine the effect of various CsA concentrations on osteoblast differentiation. According to different concentration o f CsA, growth curve, apoptosis index MTT assay, ALP activation and osteocalcin secretion, in cultured NHost were analyzed. Treating osteoblasts with low concentrations of CsA increased growth rate, MTT assay activity, ALP activation and osteocalcin protein levels in a dose-dependent manner, while high concentration showed opposite results. Therefore, these results showed that low concentrations of CsA increased osteoblast differentiation, while high concentrations elicited an opposite response, showing inhibition of CsA on osteoblast differentiation. It suggested that different CsA concentrations might play in regulating NHost differentiation, and its specific activation of lower concentration will represent a viable anabolic therapy for bone resorption disease in future.
키워드
Cyclosporin A; Differentiation; NHost
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